OECD 498: In Vitro Phototoxicity Test Using the RhE Method

OECD 498: In Vitro Phototoxicity Test Using the RhE Method

OECD 498 is a standardized in vitro test method designed to assess the phototoxic potential of substances on human skin. This test evaluates the potential for a chemical to cause skin damage upon exposure to ultraviolet (UV) light, which is especially important for the safety assessment of cosmetic products, pharmaceuticals, and other chemicals that may come into contact with the skin.

The test utilizes reconstructed human epidermis (RhE) models that accurately replicate the structure and function of human skin, allowing for a more relevant assessment of phototoxic effects. During the assay, the test substance is applied to the RhE model, followed by exposure to UV light. The subsequent effects on cell viability, inflammation, and overall skin integrity are then evaluated.

At PA Biotechnology, we conduct the OECD 498 test to provide reliable data on the phototoxicity of products and substances. Our testing services help manufacturers ensure the safety of their formulations, allowing for informed decision-making in product development and regulatory compliance. By employing the OECD 498 method, we support the creation of safe, effective products while minimizing risks associated with UV exposure in consumers.